Background: This study aimed to assess the impact of macular photocoagulation on Visual Field and nerve fiber layer thickness in patients undergoing treatment for diabetic macular edema. Material and Methods: A prospective interventional case series was conducted, involving 26 eyes of patients with a history of diabetes and clinically significant macular edema eligible for macular photocoagulation. All participants underwent 10-2 and 24-2 Humphrey Visual Field Test using the Swedish Interactive Thresholding Algorithm (SITA) standard strategy, as well as optic nerve and macular optical coherence tomography (OCT) before and six months after macular laser photocoagulation. Changes in Visual Field, peripapillary, and macular nerve fiber layer thickness were compared pre-and post-photocoagulation. Results: The study included patients with a mean age of 57. 60 ±,8. 99 (range 33-73) years. No statistically significant changes were observed in mean deviation, pattern standard deviation, and foveal threshold during the 10-2 and 24-2 Visual Field tests after photocoagulation, except for the pattern standard deviation in the 10-2 test. Conclusion: The findings of this study indicate that macular laser photocoagulation does not have a significant impact on the Visual Field.